Abcam, ab263285, Human NT5C knockout HEK-293T cell lysate

Size: 1Kit
NT5C KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 25 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 25 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NT5C, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NT5C also known as dNT-1 is a cytosolic enzyme with a molecular weight of approximately 41 kDa. It functions by dephosphorylating nucleoside monophosphates into nucleosides and its activity is important for nucleotide metabolism in cells. NT5C is expressed in various tissues including liver brain and skeletal muscle enabling these tissues to efficiently manage nucleotide balance.
Biological function summary
NT5C plays a role in maintaining cellular nucleotide pools impacting DNA and RNA synthesis. The enzyme is not part of a larger protein complex and primarily functions independently in nucleotide salvage pathways. Its activity influences the availability of nucleosides which are necessary for cellular replication and repair processes.
Pathways
NT5C contributes to purine and pyrimidine metabolism. It is involved in the nucleotide salvage pathways collaborating with other enzymes like adenosine deaminase and cytidine deaminase. These pathways play essential roles in recycling nucleotides and maintaining the nucleoside balance critical for cellular function.
NT5C has implications in cancer and neurological diseases. Abnormal activity of NT5C might contribute to nucleotide imbalances observed in certain cancers. Its role in dephosphorylation in the brain links it with neurodegenerative conditions potentially involving proteins like adenosine kinase. Understanding its function may offer insights into therapeutic strategies for these diseases.