Abcam, ab263286, Human OGFR knockout HeLa cell lysate

Size: 1Kit
OGFR KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-OGFR, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Opioid Growth Factor Receptor (OGFr) is a protein that significantly influences cellular growth and regulation. Also known as zeta opioid receptor OGFr has an approximate molecular mass of 73 kDa. Researchers find this receptor in various tissues but it is most highly expressed in the brain and eyes. OGFr is a cytoplasmic and nuclear receptor indicating its versatile roles in different cellular compartments. It binds to the Opioid Growth Factor (OGF) also recognized as [Met^5]-enkephalin.
Biological function summary
OGFr regulates cell proliferation impacting both normal tissue maintenance and healing processes. This receptor functions as part of a complex with OGF thereby playing a fundamental part in the negative regulation of cell proliferation. By binding OGF OGFr enters the nucleus and modulates transcription events that influence cell cycle progression. OGFr activity affects diverse cells including those in corneal and epidermal tissues emphasizing its role in cellular homeostasis and repair mechanisms.
Pathways
OGFr integrates into the OGF-OGFr axis a pathway critical for the control of cell growth. This axis plays an important role in the regulation of both DNA synthesis and the cell cycle. OGFr interacts closely with the p16 and p21 proteins which are essential to the pathway's function in cell cycle modulation. The OGF-OGFr axis represents an important biological system that provides a means for cells to regulate their proliferation rate through receptor-ligand interaction.
Researchers have found that OGFr plays a significant part in the growth of various types of cancers such as squamous cell carcinoma and pancreatic cancer. Altered expression or function of OGFr may correlate with abnormal cell proliferation and tumor development. Moreover OGFr's relationship with proteins such as OGF and p21 highlights its potential role in therapeutics for these disease conditions. As targeting this pathway can modulate cell growth it presents a possible avenue for cancer treatment strategies.