Abcam, ab263287, Human OTOP3 knockout A549 cell lysate

Size: 1Kit
OTOP3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.,
Disease:Carcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-OTOP3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The OTOP3 protein also known as otopetrin 3 functions as an important component in the mechanosensory system. It is an ion channel thought to contribute significantly to the transduction of mechanical signals into cellular responses. OTOP3 has a calculated mass of approximately 65 kDa. Researchers have observed its expression in tissues like the cochlea and vestibular system indicating a role in auditory and equilibrium processes.
Biological function summary
The OTOP3 protein serves critical roles in sensory perception particularly in the detection of gravitational and vibrational stimuli. It is not typically known to be part of a larger protein complex but operates as an individual unit. The spatial distribution in the inner ear suggests a function in the maintenance of otoconia the calcium carbonate crystals necessary for balance.
Pathways
OTOP3 participates in the sensory transduction pathways. It plays an important part in mechanotransduction where it works alongside proteins like OTOP1. In pathways such as these OTOP3 possibly facilitates ionic exchange across sensory cell membranes contributing to the depolarization events required for signal generation.
Mutations or dysfunctions in the OTOP3 protein have potential links to balance impairments such as vertigo and certain types of hearing loss. Studies hint at interactions with proteins like otogelin which could amplify these effects if defective. The relationship of OTOP3 with otic disorders proposes a significant area for further investigation to understand sensory deficiencies better.