Abcam, ab263289, Human OTUD4 (HIN-1) knockout HeLa cell lysate

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OTUD4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and Insertion of the selection cassette in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and Insertion of the selection cassette in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-OTUD4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
OTUD4/HIN-1 also known as ovarian tumor domain-containing protein 4 is known for its deubiquitinating enzyme activity. This protein shows a molecular mass of approximately 135 kDa. It is expressed in various tissues with significant levels observed in the brain and kidneys. OTUD4 plays a role in regulating protein degradation by removing ubiquitin impacting cellular processes such as protein turnover cell cycle regulation and signaling pathways.
Biological function summary
The OTUD4 protein significantly influences cellular homeostasis and stress response. It participates in the protein quality control system as part of a complex that regulates the ubiquitin-proteasome system. This protein ensures the proper folding and degradation of misfolded proteins impacting cellular health and function. OTUD4 forms interactions with other proteins highlighting its role in modulating cellular stress responses and apoptosis.
Pathways
OTUD4 has connections with both the ubiquitin-proteasome and DNA damage response pathways. It participates in the modulation of these pathways ensuring that damaged or misfolded proteins do not accumulate. OTUD4 directly interacts with proteins such as ubiquitin-specific proteases and E3 ligases influencing apoptosis and stress response. These interactions reveal its involvement in cellular decisions related to repair survival or apoptosis.
OTUD4 shows associations with neurodegenerative diseases and certain cancers. Dysregulation in its function can relate to protein aggregation in neurodegenerative conditions with its expression affecting pathways related to proteins like Parkin and UCH-L1. In cancers changes in OTUD4 expression can impact tumor growth and survival pointing towards its potential for therapeutic targeting and biomarker use in these disorders.