Abcam, ab263290, Human OXA1L knockout HEK-293T cell lysate

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OXA1L KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-OXA1L, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
OXA1L also known as mitochondrial inner membrane protein OXA1L functions mechanically in the insertion and assembly of proteins in the mitochondrial inner membrane. This protein with a mass of approximately 45 kDa localizes to the mitochondria and exhibits broad tissue expression. OXA1L plays a role in protein translocation specifically contributing to the insertion of multi-spanning membrane proteins coming from the mitochondrial ribosomes.
Biological function summary
OXA1L facilitates the integration of proteins into the mitochondrial inner membrane essential for maintaining mitochondrial function and biogenesis. OXA1L operates as part of a larger complex that assists protein assembly and stabilization. Such interactions highlight its role in mitochondrial respiratory chain assembly necessary for maintaining cellular energy balance.
Pathways
OXA1L integrates specifically into the pathway of mitochondrial oxidative phosphorylation. This target works alongside important proteins like COX1 and COX2 shaping the proper assembly of components of the electron transport chain. Additionally OXA1L interacts with the mitochondrial import machinery vital for maintaining the efficiency of protein import pathways that regulate cellular respiration.
Mutations or dysregulation of OXA1L correlate with mitochondrial disorders and neurological diseases including sensorineural hearing loss and mitochondrial complex I deficiency. NDUFAF2 involved in complex I assembly shows interaction with OXA1L in these conditions highlighting a shared pathway that can lead to disrupted energy production and neurological symptoms.