Abcam, ab263292, Human PDAP1 knockout HEK-293T cell lysate

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PDAP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PDAP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDAP1 also known as PDGFA-associated protein 1 is a protein with mass around 34 kDa. It associates with the A chain of platelet-derived growth factor (PDGFA) to modulate its activity. PDAP1 expression occurs widely across human tissues including high levels in the liver and pancreas which suggests its diverse roles within the body. Its interaction with growth factors like PDGFA points towards its involvement in cellular growth and development processes.
Biological function summary
The PDAP1 protein interacts with several cellular components to regulate cell proliferation and differentiation. Although not a component of a large protein complex its action still critically influences the PDGFA signaling pathway impacting cell growth. The modulation of PDGFA activity by PDAP1 plays an important role in controlling the positive effects of growth factors ensuring balanced cellular functions.
Pathways
PDAP1 features in the PDGF signaling pathway which is essential for various cellular processes like development and tissue repair. This signaling pathway influences physiological processes such as cell movement growth and survival through its interaction with PDGFA and other proteins such as PDGFR (platelet-derived growth factor receptor). These interactions highlight PDAP1's functional role in maintaining cellular and tissue homeostasis through well-regulated signaling cascades.
Altered PDAP1 activity links to certain pathological conditions including fibrosis and cancer. In fibrosis PDAP1’s relation to PDGFA influences excessive tissue scarring impacting organ function. In oncogenesis its modulation of growth factor signaling can lead to uncontrolled cell growth and tumor development. The association with key proteins like PDGFR in these conditions suggests that PDAP1 impacts disease progression and presents potential therapeutic targets for interventions.