Abcam, ab263293, Human PDIA5 knockout HeLa cell lysate

Size: 1Kit
PDIA5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PDIA5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein disulfide-isomerase A5 (PDIA5) also known as PDIR serves as an enzyme that catalyzes the formation breakage and rearrangement of disulfide bonds. It weighs approximately 58 kDa. Researchers find PDIA5 expressed in the endoplasmic reticulum especially in tissues with high secretory activity like the pancreas and liver. It participates in protein folding and maintaining cell function by ensuring proper protein structure through disulfide bond formation.
Biological function summary
PDIA5 has important roles in regulating redox homeostasis and protein quality control within the endoplasmic reticulum. It does not operate alone; it functions as part of multi-protein complexes engaging with other chaperones to assist in folding newly synthesized proteins. These interactions prevent protein aggregation and accumulation of misfolded proteins protecting cells from stress.
Pathways
PDIA5 integrates into the unfolded protein response (UPR) and endoplasmic reticulum-associated degradation (ERAD) pathways. It interfaces with other proteins like BiP/GRP78 which enhances its chaperone activity during cellular stress and EDEM1 a protein involved in recognizing misfolded glycoproteins. These pathways work together to maintain cellular homeostasis by managing protein load and degradation processes.
Research associates PDIA5 with various conditions including neurodegenerative diseases and cancer. Misfolding and aggregation of proteins linked to PDIA5 malfunction can contribute to disorders like Alzheimer's disease. Moreover PDIA5 may influence cancer progression by interacting with proteins like CALR which also participates in cell stress responses. Understanding PDIA5 functions helps in developing potential therapeutic strategies for these conditions.