Abcam, ab263294, Human PEX5 (PER3) knockout HEK-293T cell lysate

Size: 1Kit
PEX5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon3.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon3.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PEX5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PEX5 (Peroxisomal Biogenesis Factor 5) also referred to as peroxin-5 functions as a cytosolic receptor for peroxisomal targeting signal 1 (PTS1) proteins. With a mass of approximately 71 kDa PEX5 exhibits widespread expression in various tissues. It facilitates the transport of proteins into peroxisomes which are cellular organelles critical for lipid metabolism and the detoxification of reactive oxygen species. PEX5 achieves this by binding to PTS1-tagged proteins in the cytosol and guiding them to the peroxisomal membrane.
Biological function summary
The PEX5 protein plays a significant role in peroxisomal protein import by participating in a transient multi-protein transport complex at the peroxisome membrane. This complex ensures that imported proteins are released into the peroxisomal matrix. PEX5 interacts closely with PEX14 a peroxisomal membrane protein to facilitate the docking of PTS1-carrying proteins. PEX5 is indispensable for maintaining normal peroxisomal function and integrity as it ensures that specific enzymes reach their destination to carry out metabolic processes.
Pathways
PEX5 interacts within the broader lipid metabolism and reactive oxygen species detoxification pathways. It contributes to the proper functioning of enzymes involved in beta-oxidation of fatty acids and plasmalogen synthesis. PEX5 operates in conjunction with the PEX7 receptor which handles the PTS2 pathway helping to manage the trafficking of enzymes important for peroxisomal operations thereby integrating lipid metabolism with cellular homeostasis.
Mutations in the PEX5 gene often result in peroxisome biogenesis disorders such as Zellweger syndrome which is part of Zellweger spectrum disorders. These conditions manifest due to defects in peroxisomal function leading to neurological impairment and metabolic dysregulation. PEX14 has been identified as a related protein as it cooperates with PEX5 in the peroxisomal matrix protein import pathway. Understanding PEX5’s function and interactions is essential for developing strategies to address these genetic disorders.