Abcam, ab263295, Human PIGT knockout HEK-293T cell lysate

Size: 1Kit
PIGT KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 5 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 5 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PIGT, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIGT (Phosphatidylinositol Glycan Anchor Biosynthesis Class T) plays a mechanical role in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. Also known as PIG-T this protein is part of the GPI transamidase complex and has a molecular mass of approximately 68 kDa. It resides in the endoplasmic reticulum where it supports the attachment of various proteins to the cell membrane through GPI anchoring. PIGT is ubiquitously expressed across different human tissues indicating its fundamental role in cellular physiology.
Biological function summary
PIGT acts as a subunit within the GPI transamidase complex that enhances the transfer of pre-assembled GPI units to proteins. This activity anchors proteins to the cell surface which is necessary for their stability and function. The GPI anchor allows effective signaling adhesion and protection of proteins impacting many cellular processes. The interdependency with other subunits such as PIGA PIGK and PIGS ensures the complete function of the GPI transamidase complex.
Pathways
PIGT remains key within the GPI-anchor biosynthesis pathway playing a critical role in cell surface protein localization. It interacts with other members like PIGU and GAA1 within this pathway forming interactions necessary for proper transit and attachment of GPI-anchored proteins. The functionality of the GPI-anchor pathway directly influences cellular communication through pathways like signal transduction which are essential for different biological processes.
Mutations or deficiencies in PIGT link to Paroxysmal Nocturnal Hemoglobinuria (PNH) and Early Infantile Epileptic Encephalopathy (EIEE). PNH involves defective GPI-anchored proteins affecting the regulation of cell membrane stability while EIEE relates to neurological complications due to disturbed protein functioning. PIGT connects with other proteins like PIGM and PIGL contributing to the disorders' pathophysiology when disruptions in GPI-anchor biosynthesis occur.