Abcam, ab263299, Human PLA2G12A (Phospholipase A2 XII) knockout HeLa cell lysate

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PLA2G12A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PLA2G12A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phospholipase A2 XII also known as PLA2G12A is an enzyme that catalyzes the hydrolysis of the sn-2 acyl bond in glycerophospholipids liberating free fatty acids and lysophospholipids. It is part of the phospholipase A2 family but lacks conventional enzymatic activity seen in other members. PLA2G12A has a molecular mass of approximately 19 kDa. This enzyme is secreted and expressed in various tissues including the pancreas and macrophages suggesting roles in different physiological contexts.
Biological function summary
The enzyme belongs to the large group of secreted phospholipases A2 that contribute to diverse biological processes despite its low catalytic activity. It does not form part of a complex but often interacts with other molecules during lipid metabolism. Also it participates in lipid mediator production which impacts inflammation and host defense mechanisms. Its presence in immune cells indicates a potential involvement in immune responses and tissue homeostasis.
Pathways
PLA2G12A is essential in the arachidonic acid metabolism pathway and inflammatory signaling pathways. It connects with other proteins such as secretory phospholipase A2 and cytosolic phospholipase A2. The enzyme's action leads to the release of lipid mediators that modulate inflammatory pathways highlighting its regulatory role in these networks. It influences the balance and interplay between the production and degradation of lipid mediators important for biological signaling.
Research points to PLA2G12A's involvement in inflammatory diseases and metabolic disorders. In conditions like arthritis elevated levels of PLA2G12A have been observed linking it to exacerbation of inflammation. Another connected protein cytosolic phospholipase A2 also has associations with inflammatory diseases and amplifies the role of PLA2G12A in inflammation. Understanding its function may provide further insights into therapeutic targets for reducing inflammation in such disorders.