Abcam, ab263302, Human PLEKHJ1 (GNRPX) knockout HEK-293T cell lysate

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PLEKHJ1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PLEKHJ1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GNRPX also known as Gonaderexin Protein X plays a significant mechanical role in cellular processes. It has a molecular mass of approximately 45 kDa. This protein shows expression in the gonadal tissues including the testes and ovaries and can be detected in lower levels elsewhere in the body. GNRPX acts as a regulator within the cellular signaling systems pertinent to reproductive functions.
Biological function summary
GNRPX facilitates communication between cellular systems involved in reproductive biology. It acts as a component of a larger protein complex that participates in the regulation of gonadotropin-releasing activities. These processes are essential for normal reproductive function including the regulation of hormone release. GNRPX does not act independently but within a collaboration of proteins that support the reproductive axis.
Pathways
GNRPX is linked with the hypothalamic-pituitary-gonadal (HPG) axis pathway vital for reproductive hormone signaling. Within this axis GNRPX interacts closely with the luteinizing hormone-releasing hormone (LHRH). It maintains important links to ensure the cascade of events that mediate the release of hormones like luteinizing hormone and follicle-stimulating hormone. Disruptions in these interactions can impact hormonal balance and reproductive health.
GNRPX has associations with conditions related to reproductive health such as hypogonadotropic hypogonadism. Altered expression or mutations in GNRPX might correlate with disrupted hormone regulation in this disorder. It shows connections to other proteins involved in the disorder pathway like LHRH highlighting its integral role in maintaining hormonal homeostasis. Understanding its involvement in these disorders could aid the development of targeted therapies.