Abcam, ab263306, Human POGK knockout HeLa cell lysate

Size: 1Kit
POGK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 2 bp deletion in exon4.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 2 bp deletion in exon4.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-POGK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
POGK also known as Pogo transposable element with KRAB domain functions as a transcriptional regulator. It plays a role in chromatin modification by interacting with histones. The protein has an approximate mass of 73 kDa. POGK is found in various tissues with higher expression observed in the testis placenta and certain brain regions indicating its involvement in diverse biological processes.
Biological function summary
POGK participates in regulating gene expression through its transcriptional repression activity. The protein forms part of a larger repressive complex known as the KRAB-ZFP/KAP1 network. This complex engages with histone methyltransferases to modify histones which results in transcriptional silencing of target genes. POGK thereby contributes to maintaining chromatin structure and regulating genomic stability.
Pathways
POGK engages in epigenetic regulation pathways influencing gene silencing and chromatin remodeling. It works closely with members of the KRAB-ZFP family and KAP1 key regulators in these pathways. POGK's activity impacts the silencing of endogenous retroviruses important for normal developmental processes and cellular differentiation.
POGK links to certain diseases such as cancer and neurological disorders. Aberrations in POGK expression or function may lead to dysregulated gene silencing contributing to oncogenesis. In neurological conditions POGK's role in chromatin organization may affect neural gene expression influencing diseases like schizophrenia. KRAB-ZFPs and KAP1 also associate with these conditions highlighting their interconnected roles in disease manifestation.