Abcam, ab263326, Human RBM42 knockout HEK-293T cell lysate

Size: 1Kit
RBM42 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 40 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 40 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RBM42, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RBM42 also known as RNA-binding motif protein 42 functions as an RNA-binding protein. It belongs to a family characterized by its ability to interact with RNA molecules. RBM42 has a molecular mass of around 40 kDa. Expression occurs mainly in the nucleus of cells indicating its role in post-transcriptional genetic processes. Studies found high expression levels in tissues like the brain and testis suggesting specialized functions in these regions.
Biological function summary
RBM42 influences the splicing and processing of mRNA transcripts. It interacts directly with pre-mRNA molecules implicating it in the regulation of gene expression. This protein does not function in isolation; it becomes a part of complexes with other splicing factors which cooperate to ensure accurate splicing. These interactions help maintain cellular processes that rely on correctly processed RNA.
Pathways
RBM42 interacts significantly in mRNA processing and splicing pathways. It influences pathways like the spliceosome cycle important for generating diverse protein isoforms necessary for cellular function. RBM42 works along with other RNA-binding proteins like RBM39 enhancing the splicing repertoire within cells. These interactions streamline the gene expression processes ultimately controlling how cells respond to different physiological stimuli.
Improper RBM42 function links to neurodegenerative disorders and cancers. Its malfunction can affect the alternative splicing of genes important for brain health contributing to conditions like Alzheimer's disease through connections with proteins like TDP-43. Additionally dysregulation may lead to abnormal cell proliferation and survival linked to cancers where RBM42 interacts with factors involved in cell cycle regulation altering cellular growth and division.