Abcam, ab263338, Human SCAND1 knockout HeLa cell lysate

Size: 1Kit
SCAND1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SCAND1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SCAND1 also known as SCAN domain-containing protein 1 has a molecular mass of approximately 20 kDa. Mechanically SCAND1 functions as a transcriptional regulator utilizing its SCAN domain to mediate protein-protein interactions and influence gene expression. Expression of SCAND1 occurs mainly in the nucleus across various cell types indicative of its role in controlling gene transcription.
Biological function summary
SCAND1 acts as a coordinator in cellular transcription machinery by interacting with other proteins. It often forms part of multiprotein complexes that govern chromatin remodeling and gene transcription. Through its interactions SCAND1 can influence the expression of genes that are critical in cell cycle regulation and development. These interactions contribute to cellular homeostasis and equilibrium.
Pathways
SCAND1 fits into gene transcription and chromatin remodeling circuits. Specifically it engages in interactions within the Notch signaling pathway and the Wnt signaling pathway. These pathways involve cooperation with other proteins like Notch receptors and beta-catenin which are pivotal in developmental processes and cell fate determination. SCAND1 modulates transcriptional responses that are delicate to environmental and intracellular cues.
SCAND1 shows a connection with cancer and neurological disorders. Alterations in SCAND1 expression or function can disturb normal transcriptional regulation contributing to tumorigenesis. In cancer SCAND1 may interact with proteins like p53 a well-known tumor suppressor protein. Misregulation of SCAND1 has been observed in some neurological disorders where its interaction with transcription factors is altered affecting neural functions. Understanding SCAND1's role in these diseases could open new avenues for therapeutic interventions.