Abcam, ab263342, Human SEPHS1 (Selenophosphate synthetase 1) knockout HEK-293T cell lysate

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SEPHS1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SEPHS1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Selenophosphate synthetase 1 also known as SEPHS1 or SPS1 catalyzes the synthesis of selenophosphate from selenide and ATP. It plays a critical role in the production of selenium-containing biomolecules. The mass of SEPHS1 approximates 46 kDa. SEPHS1 is expressed in various tissues including the liver and kidneys suggesting its involvement across multiple biological processes. Researchers often recognize SEPHS1 for its activity in selenium metabolism.
Biological function summary
The protein is involved in the synthesis of selenoproteins important for cellular antioxidant defense and redox homeostasis. Selenophosphate synthetase 1 functions as part of a multi-enzyme complex essential for the biosynthesis of selenocysteine the 21st amino acid which is then incorporated into selenoproteins. These selenoproteins include glutathione peroxidases and thioredoxin reductases which are important for maintaining cellular integrity.
Pathways
The protein participates actively in the selenocysteine biosynthesis and recycling pathway ensuring adequate supply of selenophosphate needed for selenoprotein production. Within this pathway SEPHS1 closely associates with several enzymes like selenocysteine synthase and selenocysteine tRNA (tRNASec) which facilitate the incorporation of selenocysteine into proteins. These interactions highlight the interconnected role of SEPHS1 in maintaining proper cellular functions through selenoproteins.
SEPHS1 is linked to deficiency-related conditions such as Keshan disease a type of heart disorder due to low selenium levels and certain thyroid dysfunctions linked to disrupted selenoprotein synthesis. Disruption of SEPHS1 function impairs selenoprotein production impacting proteins like glutathione peroxidase and thioredoxin reductase which are vital in preventing oxidative damage hence making SEPHS1 significant in understanding and potentially mitigating such disorders.