Abcam, ab263381, Human TBRG4 knockout HEK-293T cell lysate

Size: 1Kit
TBRG4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TBRG4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TBRG4 also known as FAM195A or BRG1-Associated Factor-53A is a protein with a molecular mass of approximately 144 kDa. It participates in the regulation of cellular homeostasis and function by interacting with other proteins in the cell. Expression of TBRG4 is not limited to a specific tissue as it can be detected in multiple tissues across the body including brain and immune cells. Due to its versatile expression TBRG4 likely takes part in general cellular mechanisms across different cell types.
Biological function summary
TBRG4 influences several cellular processes through its involvement in protein interactions. It is an integral part of large molecular complexes that regulate various gene expression and remodeling of chromatin. TBRG4 often functions in concert with other components to ensure accurate modulation of transcriptional activity. Complex formation affects both direct regulation of target genes and the structural rearrangements necessary for cellular homeostasis.
Pathways
The protein plays a role in the cellular response to stress and metabolic signaling. It acts within the mTOR signaling pathway which is central to the regulation of growth and metabolism. TBRG4 interacts with other proteins like Raptor and mLST8 to modulate mTOR complex functions. Additionally it is related to the PKC signaling pathway where it may indirectly influence cellular responses to external stimuli through modulation of other participating proteins.
TBRG4 connects to both cancer and neurodegenerative diseases due to its role in cell growth and stress response. Abnormal expression or malfunction of TBRG4 may contribute to the progression of certain cancers reflecting its interaction with proteins like p53 in tumor suppression pathways. In neurodegenerative conditions alterations in TBRG4-related pathways might exacerbate pathological features particularly through dysregulated signaling mechanisms involving proteins such as Tau.