Abcam, ab263386, Human TMEM106C knockout HEK-293T cell lysate

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TMEM106C KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 106 bp deletion in exon2 and 70 bp insertion in exon2 and Insertion of the selection cassette in exon2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 106 bp deletion in exon2 and 70 bp insertion in exon2 and Insertion of the selection cassette in exon2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMEM106C, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMEM106C also known as Transmembrane Protein 106C is a protein that plays a role in cellular processes. It has a molecular mass of approximately 29 kDa. This protein is generally observed to be expressed in a variety of tissues but specific expression patterns may depend on the cellular context and physiological conditions. TMEM106C localizes mainly to the cell membrane which suggests its involvement in transmembrane signaling or transport mechanisms.
Biological function summary
The protein can participate in processes related to cellular homeostasis and signaling pathways. Although it is not widely characterized as part of a larger complex the protein likely interacts with other membrane-associated proteins to mediate its effects. Potential biological activities may include regulatory mechanisms that ensure proper cellular function and communication.
Pathways
TMEM106C interacts with components involved in cellular signaling and communication pathways. It may be involved in pathways like the PI3K-Akt signaling pathway which is important for regulating cell cycle and survival. TMEM106C may also share relationships with proteins such as Akt and PI3K which are central to these pathways contributing to proliferative and survival signaling cascades.
TMEM106C is associated with neurodegenerative and metabolic conditions. For instance alterations in its expression or function might play a role in Alzheimer's disease where other proteins like tau are also involved. Additionally metabolic disorders such as diabetes may also highlight the role of TMEM106C through its connection with insulin signaling-related pathways influencing glucose metabolism and homeostasis.