Abcam, ab263419, Human WDR34 knockout HEK-293T cell lysate

Size: 1Kit
DYNC2I2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 5 bp deletion in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 5 bp deletion in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-DYNC2I2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WDR34 also known as WD repeat domain 34 is a protein with a mass of approximately 64 kDa. This protein contains WD repeat domains which facilitate protein–protein interactions. WDR34 is mostly expressed in a range of tissues including the heart skeletal muscle and kidney. It functions mechanically as a part of cellular mechanisms that involve signaling pathways essential for normal cell functioning.
Biological function summary
WDR34 plays a significant role in the regulation of ciliary assembly and maintenance. It forms a component of the intraflagellar transport (IFT) complex which is important for the functioning of primary cilia. Cilia are hair-like structures on cell surfaces that are important for signal transduction and environmental sensing. WDR34 contributes to the anterograde transport within cilia a process necessary for the proper assembly and function of the cilia structures. This involvement highlights its importance in maintaining cellular homeostasis through ciliary functions.
Pathways
The activity of WDR34 integrates into the Hedgehog signaling pathway which is critical in cell differentiation tissue patterning and embryonic development. It interacts with other IFT proteins such as IFT88 to modulate signal transduction processes within cilia. In the context of its role in ciliary transport WDR34 influences the activation and distribution of signaling molecules necessary for pathway functionality. The Hedgehog signaling is closely linked with various developmental processes indicating WDR34’s importance in proper physiological development.
Mutations in WDR34 are associated with several ciliopathies including Jeune asphyxiating thoracic dystrophy (JATD) and short rib-polydactyly syndrome (SRPS). These are genetic disorders affecting skeletal development and cilia function. The disruptions in ciliary assembly and signaling stemming from WDR34 mutations elucidate a connection to these ciliopathies. Furthermore WDR34 operates alongside other ciliopathic proteins such as IFT27 in the pathogenesis of these conditions highlighting its significance in maintaining ciliary integrity and function.