Abcam, ab26350, Anti-gamma H2A.X (phospho S139) antibody [9F3]

Size: 100µg
Anti-gamma H2A.X (phospho S139) antibody [9F3] (ab26350) is a mouse monoclonal antibody detecting gamma H2A.X in Western Blot, Flow Cytometry, IHC-P . Suitable for Chinese hamster, Human, Mouse, Rat . - KO validated for confirmed specificity - Over 340 publications - Trusted since 2005
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:9F3,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Chinese hamster,
Applications:WB, IHC-P, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human H2AX phospho S139. The exact immunogen used to generate this antibody is proprietary information.P16104,
Specificity:IHC-P is only guaranteed for mouse samples.

Product details:
Anti-gamma H2A.X (phospho S139) antibody [9F3] (ab26350) is a House Mouse Monoclonal antibody and is validated for use in Flow Cyt, IHC-P, WB.
Anti-gamma H2A.X (phospho S139) antibody [9F3] (ab26350) has been cited over 343 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-gamma H2A.X (phospho S139) antibody [9F3] (ab26350) has high sensitivity and specificity.
Anti-gamma H2A.X (phospho S139) antibody [9F3] (ab26350) has 30 independent reviews from customers.
Anti-gamma H2A.X (phospho S139) antibody [9F3] (ab26350) specifically detects gamma H2A.X Phospho-S139 (UniProt ID: P16104; Molecular weight: 15kDa) and is sold in 100 ug selling sizes.
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from TCS., Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Gamma H2A.X also known as phospho H2A.X or γH2A.X is a phosphorylated form of the histone variant H2A.X. It has a molecular weight of about 14 kilodaltons and occurs primarily in they nucleus. When DNA double-strand breaks (DSBs) occur serine 139 in H2A.X undergoes rapid phosphorylation resulting in gamma H2A.X. This modification happens swiftly at the site of damage and gamma H2A.X spreads over a large chromatin area facilitating the recruitment of DNA repair proteins. Gamma H2A.X staining typically evaluated using gamma H2A.X immunofluorescence techniques aids in identifying the presence and extent of DNA damage.
Biological function summary
Gamma H2A.X plays a role in DNA damage response and repair. It does not operate alone; it acts as part of a complex with other repair proteins. The formation of gamma H2A.X foci at DNA damage sites creates a signal attracting repair factors that help maintain genome stability. Its interaction with MDC1 and ATM proteins exemplifies its significant role in orchestrating an effective response to DNA damage. Beyond DNA repair gamma H2A.X influences cell cycle checkpoints permitting cells to pause and repair before proceeding with division.
Pathways
Gamma H2A.X plays a pivotal role in the DNA damage response (DDR) pathway. This pathway is essential for detecting and repairing DNA lesions to uphold genomic integrity. Within the DDR pathway gamma H2A.X is closely associated with proteins such as NBS1 and BRCA1 which assist in repairing double-strand breaks. In addition gamma H2A.X is integral to the ATM-ATR signaling pathway where its activation promotes cell survival following genotoxic stress by signaling for damage repair or triggering apoptosis.
Gamma H2A.X has connections to cancer and neurodegeneration. Aberrant DNA repair pathways often indicated by persistent gamma H2A.X signals correlate with tumor formation and progression. For instance a failure to repair DNA damage effectively can lead to mutations that drive cancer development. Gamma H2A.X also links to neurodegenerative diseases where dysregulated DNA repair contributes to neuronal cell death. Proteins like p53 which regulate cell cycle and apoptosis further connect to gamma H2A.X bridging its role in disease pathogenesis.