Abcam, ab264039, Anti-heavy chain Myosin antibody [F1.652] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal MYH3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:F1.652,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Mouse, Rat,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Cross-reactivity with this protein has not been confirmed experimentally.

Product details:
ab264039 is the carrier-free version of
ab264038
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Heavy chain Myosin also known as MYH3 is a protein involved in muscle contraction. Alternative names for this protein include Myosin heavy chain and MyHC with a molecular mass of approximately 223 kDa. This protein is primarily expressed in skeletal muscle tissues during fetal development. The heavy chain myosin is a part of the larger family of myosin proteins which are motor proteins found in most eukaryotic cells.
Biological function summary
Heavy chain Myosin functions to convert chemical energy from ATP into mechanical energy facilitating muscle contraction. It serves as the backbone for thick filaments playing a significant role in muscle fiber composition. Myosin heavy chain forms a complex with actin filaments and this actin-myosin interaction is essential for muscle contraction and force generation. This protein can associate with different myosin light chains and regulatory proteins which modulate its activity.
Pathways
Heavy chain Myosin is an important player in the muscle contraction pathway. This pathway is critical for muscle development and maintenance influencing how muscles respond to stimuli. Actin and tropomyosin are vital collaborators in this process with the interaction between actin and MyHC being central to contraction. The troponin complex acts as a regulatory unit within the pathway controlling the exposure of active sites on actin filaments.
Mutations in the MYH3 gene can cause conditions like Freeman-Sheldon syndrome and distal arthrogryposis type 2A. These disorders are characterized by joint contractures and muscle abnormalities. MYH3 mutations alter the normal function of the myosin protein affecting muscle development. Additionally heavy chain Myosin interacts with other muscle-related proteins such as actin and myosin-binding protein C linking it to various skeletal muscle disorders.