Abcam, ab264116, Anti-Rpa2 (phospho S4 + S8) antibody

Size: 100µg
Rabbit Polyclonal RPA32/RPA2 phospho S4 + S8 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPA2 phospho S4 + S8.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IP, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RPA2 phospho S4 + S8. The exact immunogen used to generate this antibody is proprietary information.P15927

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Replication protein A2 (Rpa2) is an essential component of the DNA replication and repair processes. Also known as RPA32 or RFA2 Rpa2 is a subunit of the heterotrimeric complex known as Replication Protein A (RPA) which plays an important role in stabilizing single-stranded DNA. It has a molecular mass of approximately 32 kDa and is ubiquitously expressed across various cell types indicating its fundamental role in cellular maintenance and function. Rpa2 is primarily located in the nucleus where it contributes to the regulation of DNA metabolism.
Biological function summary
Rpa2 is part of the RPA complex that participates in DNA replication repair and recombination. This protein binds to single-stranded DNA protecting it from nucleases and preventing secondary structures that could form during replication or repair. In addition to its structural role Rpa2 facilitates the recruitment and stabilization of other essential proteins involved in DNA polymerization and repair ensuring the integrity of genetic material during cell division. The interaction of Rpa2 with various partners allows the cell to efficiently address DNA damage through coordinated signaling and repair mechanisms.
Pathways
Rpa2 is central to both the DNA replication and damage response pathways. It interacts closely with other proteins such as ATR and RAD51 which play important roles in the cellular response to DNA damage. In the replication pathway Rpa2 helps form pre-replication complexes by stabilizing and protecting the single-stranded DNA needed for the replication machinery. In the DNA damage response Rpa2 facilitates checkpoint signaling and repairs through its interactions with ATR which enables the cell to pause the cycle and perform necessary repairs for maintaining genomic stability.
Disruptions in Rpa2 function correlate with genomic instability contributing to cancer and genetic disorders like xeroderma pigmentosum. Its role in maintaining DNA replication fidelity makes it a critical factor in cancer where mutations or dysregulations may promote tumorigenesis by allowing unchecked cell proliferation. Rpa2's involvement with proteins such as RAD51 is significant in homologous recombination repair a pathway frequently compromised in cancers leading to increased mutation rates and disease progression. Understanding Rpa2's role in these contexts can aid in the development of targeted therapies and enhance prognosis for affected individuals.