Abcam, ab264330, Anti-FIP antibody

Size: 100µg
Rabbit Polyclonal FIP antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human USF2 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human USF2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q15853

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FIP also known as Family Interacting Protein or Optineurin is a multifunctional protein with a mass of approximately 66 kDa. It is expressed in various tissues with the highest levels in the heart brain and skeletal muscles. FIP functions mechanically as a scaffold protein interacting with a variety of cellular partners to regulate numerous processes. It contains multiple domains that allow it to bind to other proteins and influence cellular signaling pathways.
Biological function summary
FIP impacts cell signaling vesicle trafficking and inflammation. It often participates as a part of larger protein complexes aiding in the fine-tuning of cellular responses. FIP acts as an important mediator in autophagy assisting in the degradation of damaged cellular components. The protein also modulates the inflammatory response by interacting with signaling molecules impacting pathways like NF-kB signaling and mitigating excessive inflammation.
Pathways
FIP influences cellular homeostasis and immune responses. It plays a significant role in the NF-kB signaling pathway which regulates immune responses and cell survival. FIP forms interactions with proteins such as RIPK1 and ubiquitin chains affecting downstream signaling events. Additionally it participates in the autophagy pathway collaborating with proteins like LC3 to mediate the clearance of cellular debris which is critical for maintaining cellular health.
Aberrations in FIP function have been associated with glaucoma and amyotrophic lateral sclerosis (ALS). In glaucoma mutations in FIP are connected to altered autophagic processes impacting optic nerve health. The protein also interacts with TBK1 another factor linked to variations in immune and inflammatory responses contributing to the disease. Furthermore in ALS dysregulation in FIP’s regulatory role in autophagy and inflammation might accelerate neuronal degeneration highlighting its potential as a target for therapeutic intervention.