Abcam, ab264519, Anti-Tubulin antibody [YL1/2] - BSA and Azide free

Size: 100µg
Rat Monoclonal Alpha-tubulin 1 acetyl K40 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:YL1/2,
Isotype:IgG2a,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:The YL1/2 monoclonal epitope has been mapped to the last 8 residues (GEEEGEEY) at the carboxy terminus of alpha tubulin when tyrosinated (PubMed IDs: 6415068, 6204858).

Product details:
ab264519 is the carrier-free version of
ab6160
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tubulin sometimes called DM1B is a globular protein essential for the formation of microtubules. It has a molecular mass of approximately 55 kDa. Tubulin is expressed in most eukaryotic cells and exists as two closely related subunits alpha-tubulin and beta-tubulin. These subunits polymerize to form linear microtubule structures. Anti-tubulin antibodies such as anti-tubulin Alexa 34 can detect these microtubule markers in cells allowing researchers to visualize cytoskeletal organization.
Biological function summary
This core component of microtubule assembly is important for maintaining cell shape enabling intracellular transport and segregating chromosomes during cell division. As part of a complex tubulin interacts with various microtubule-associated proteins (MAPs) that regulate its dynamic assembly and disassembly. This regulation is important for processes like axonal transport in neurons and the movement of cilia and flagella in other cell types.
Pathways
Tubulin plays a significant role in the mitotic spindle assembly part of the cell cycle. This process is vital for the accurate segregation of chromosomes to daughter cells. Tubulin interacts with the kinesin and dynein motor proteins within this pathway which are essential for intracellular transport and mitosis. Another key pathway involving tubulin is the intracellular trafficking facilitated by motor proteins which is necessary for maintaining cell homeostasis and function.
Aberrant regulation or mutations in tubulin can lead to conditions such as cancer and neurodegenerative diseases. In cancer altered tubulin dynamics contribute to uncontrolled cell proliferation. Neurodegenerative disorders such as Alzheimer’s disease show involvement with tubulin through abnormal microtubule stability often linked to the MAP tau protein. Anti-tubulin antibodies including LAMP1 can help in research related to these conditions by offering methods to study tubulin distribution and function in diseased cells.