Abcam, ab264536, Anti-PREX1 antibody [6F12] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal PREX1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:6F12,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab264536 is the carrier-free version of
ab264535
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PREX1 protein also known as phosphatidylinositol 345-trisphosphate-dependent Rac exchange factor 1 operates as a guanine nucleotide exchange factor (GEF). It facilitates the activation of the small GTPase RAC1 by exchanging GDP for GTP. The protein has a molecular mass of approximately 185 kDa. PREX1 is widely expressed in various tissues with higher levels observed in brain heart and leukocytes. Additionally it contains several distinct domains including the DH domain responsible for its GEF activity PH domain for lipid binding and DEP domain aiding in signaling pathway integration.
Biological function summary
PREX1 plays a fundamental role in modulating cellular processes such as cell migration growth and survival. It interacts with RAC1 enabling the restructuring of the actin cytoskeleton and promoting cell motility. PREX1 does not only function in isolation but is often found within complexes that influence RAC1 activity. It cooperates with proteins like PI3K (phosphoinositide 3-kinase) enhancing downstream signaling events integral to cellular function.
Pathways
PREX1 participates in pathways like PI3K/Akt and RAC1 signaling. These pathways are pivotal in regulating cellular responses to external stimuli. Interaction with PI3K leads to PREX1 activation by PIP3 which in turn activates RAC1. This association is important for signal transduction involved in cellular growth and survival. Furthermore PREX1's interaction with proteins like AKT and mTOR highlights its involvement in metabolic regulation and cellular homeostasis.
PREX1 exhibits significant correlation with cancer and cardiovascular diseases. Aberrant PREX1 expression or function often leads to altered RAC1 signaling contributing to tumorigenesis and metastasis in cancers such as breast cancer. The dysregulation of PREX1 also associates with cardiovascular conditions by influencing vascular tone and heart muscle contractility. These disease associations emphasize interactions with proteins like PI3K and RAC1 highlighting their roles in disease pathophysiology.