Abcam, ab264560, Anti-HDAC4 (pS246) + HDAC5 (pS259) + HDAC7 (pS155) antibody [EPR22997-46] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HDAC5 phospho S259 antibody. Carrier free. Suitable for Dot, IP, WB and reacts with Synthetic peptide - Human, Synthetic peptide - Mouse, Synthetic peptide - Rat, Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22997-46,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, Dot, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab264560 is the carrier-free version of
ab240643
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HDAC4 HDAC5 and HDAC7 are class IIa histone deacetylases. These proteins remove acetyl groups from lysine residues on histones leading to chromatin condensation and transcriptional repression. HDAC4 has a molecular mass of about 140 kDa HDAC5 around 122 kDa and HDAC7 approximately 107 kDa. They express predominantly in heart skeletal muscle and brain tissues. The proteins operate in both the nucleus and cytoplasm but they shuttle between these compartments regulated by phosphorylation.
Biological function summary
Histone deacetylases HDAC4 HDAC5 and HDAC7 play roles in gene expression modulation. They form complexes with transcription factors such as MEF2 (myocyte enhancer factor 2) integral in muscle differentiation processes. These HDACs influence cell cycle progression differentiation and survival by affecting the acetylation status of non-histone proteins. Their ability to silence specific gene sets makes them key regulators of cellular functions under different physiological and stress conditions.
Pathways
HDAC4 HDAC5 and HDAC7 participate in critical signaling events such as the MEF2 and NF-κB pathways. These HDACs interact with proteins in these pathways to modulate inflammation apoptosis and cardiac hypertrophy. They regulate gene expression by altering chromatin structure and functioning as transcription corepressors linking them to diverse cellular responses. Their activity connects closely with kinases like calcium/calmodulin-dependent protein kinase (CaMK) which influences their localization and activity.
HDAC4 HDAC5 and HDAC7 are associated with cardiovascular disease and certain cancers. Aberrant activity or expression of these HDACs links to pathological cardiac remodeling as they regulate genes that affect heart muscle pathology. In cancer they govern processes linked to tumor progression like cell proliferation and survival. Abnormal interactions with proteins such as p53 and MEF2 in the context of these diseases highlight their importance in pathogenesis and potential as therapeutic targets.