Abcam, ab264671, Human ZMYM3 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
ZMYM3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ZMYM3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZMYM3 also known as Zinc Finger MYM-Type Containing 3 is a protein with a molecular mass of about 155 kDa. It acts as a transcriptional regulator by binding to specific DNA sequences through its zinc-finger domains. ZMYM3 is mainly expressed in the brain and testes suggesting its role in neural and reproductive functions. The protein localizes primarily in the nucleus where it interacts with chromatin to modulate the expression of target genes.
Biological function summary
There are multiple roles of ZMYM3 in cellular processes. It is part of the CoREST complex a multiprotein scaffold involved in transcriptional repression. Through its participation in this complex ZMYM3 contributes to chromatin remodeling and epigenetic silencing of genes playing a role in maintaining the balance of gene expression necessary for normal cellular operations and development.
Pathways
There are several ways ZMYM3 impacts cellular communication and regulation. The protein is involved in the Notch signaling pathway which is important for cell differentiation proliferation and apoptotic processes. ZMYM3 influences this pathway alongside related proteins such as CoREST and LSD1. Additionally it interacts within the androgen receptor signaling pathway potentially impacting gene transcription relevant to sexual development and hormone response.
There is significant interest in the connection between ZMYM3 and cognitive impairments including X-linked intellectual disability. Mutations or dysregulation in ZMYM3 can lead to disrupted transcriptional control linking the protein to neurological disorders. Another medical condition linked to ZMYM3 is prostate cancer where it may affect androgen receptor pathways thereby implicating interactions with proteins like LSD1 and the androgen receptor itself in the disease's progression.