Abcam, ab264683, Human PI4K2B knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PI4K2B KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PI4K2B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PI4K2B also known as Phosphatidylinositol 4-kinase type 2 beta is an enzyme with a molecular mass of approximately 55 kDa. This protein plays a role in the phosphorylation of phosphatidylinositol converting it to phosphatidylinositol 4-phosphate (PI4P) an important step in the phosphatidylinositol signaling pathway. PI4K2B shows expression in a variety of human tissues with particularly high levels noted in the placenta and fibroblasts. Its activity influences the formation of different cellular membranes impacting various cellular processes.
Biological function summary
PI4K2B influences multiple cellular functions particularly those related to membrane trafficking and cell signaling. Although not part of a larger known complex PI4K2B's enzymatic production of PI4P has many downstream effects impacting endosomal dynamics and vesicular trafficking. The protein's involvement in membrane dynamics suggests important roles in regulating cell movement and recycling of membrane components affecting how cells respond to their surroundings.
Pathways
PI4K2B integrates into the broader phosphoinositide pathway that regulates cellular communication and processes. It closely interacts with proteins like AKT and RAB proteins which are essential in the regulation of vesicle transport and signal transduction. The production of PI4P by PI4K2B contributes to the modulation of the PI3K/AKT signaling pathway impacting cellular growth survival and metabolism. This pathway's complexity demonstrates PI4K2B's role in coordinating multiple biological reactions.
Studies link altered PI4K2B function to neurodegenerative diseases and cancer. Its aberrant activity influences the signaling pathways that contribute to the development and progression of these conditions. In the context of neurodegeneration PI4K2B interacts with proteins such as tau influencing the balance of endosomal transport in neurons. In cancer changes in its activity affect cellular proliferation and survival showing connections to other oncogenic proteins within signaling cascades. These implications highlight the importance of understanding PI4K2B's roles in health and disease.