Abcam, ab264698, Human TSC22D4 (TSC22 domain family, member 4) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TSC22D4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TSC22D4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TSC22 domain family member 4 (TSC22D4) also known as TSC-22R is a protein that functions largely as a transcriptional regulator. With a molecular weight approximately 31 kDa TSC22D4 resides primarily in the nucleus. This protein is expressed in a variety of tissues including liver lung and kidney. Its expression helps manage cell growth and differentiation processes.
Biological function summary
TSC22D4 plays a significant role in the regulation of gene transcription. It is part of the TSC22D family of proteins that form complexes to mediate their functions. This protein can act as a transcriptional repressor or activator which directly affects cellular proliferation and apoptosis. TSC22D4's ability to modulate other proteins' activities underlies its involvement in controlling diverse cellular processes.
Pathways
TSC22D4 interacts within the transforming growth factor-beta (TGF-β) signaling pathway and the MAPK pathway. It often partners with other proteins like SMAD2 and SMAD3 to mediate TGF-β responses that are important for cellular growth and differentiation. TSC22D4's participation in these pathways indicates its role in not only development but also in maintaining homeostasis within tissues.
TSC22D4 has a connection to cancer and fibrotic diseases. In cancers such as liver and lung carcinomas abnormal regulation of TSC22D4 expression can influence tumor growth and metastasis. Its interaction with proteins like p53 impacts cell cycle regulation and apoptosis. In fibrotic diseases altered activity of TSC22D4 affects extracellular matrix production linking it to excessive tissue scarring.