Abcam, ab264759, Human NUPR1 (p8) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
NUPR1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NUPR1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The p8 protein also known as NUPR1 is a small nuclear protein with a molecular mass of approximately 8 kDa. It mainly locates in the nucleus but can also move to the cytoplasm depending on cellular conditions. It has a regulatory role and expresses in various tissues with higher levels in response to cellular stress. Its expression tends to elevate in some cancer tissues suggesting a significant role in cellular adaptation and stress responses.
Biological function summary
The p8/NUPR1 protein functions as a transcriptional regulator involved in cell growth death and stress responses. It does not form a static complex but rather interacts with different molecular partners depending on cellular context. It regulates genes involved in cell survival and apoptosis which underlines its adaptability in cellular environments. Its dynamic role allows the modulation of several pathways in reaction to cellular stress contributing to cell homeostasis and adaptability.
Pathways
P8/NUPR1 plays an important role in stress response pathways and cell survival mechanisms. It interacts with the MAPK pathway a well-known pathway involved in cell response to stress and apoptosis. Additionally NUPR1 links to the JNK signaling pathway which modulates cell stress responses and apoptosis. In these pathways p8 acts as a coordinator between stress signals and cellular fate decisions regulating the expression of stress-induced genes and interacting with proteins such as JNK.
P8/NUPR1 relates to pancreatic cancer and neurodegenerative disorders. Its overexpression in pancreatic cancer correlates with poor prognosis regulating other proteins like p53 that influence cell cycle and apoptosis. In neurodegenerative disorders p8's role in stress responses suggests a potential involvement in neuronal cell survival and apoptosis connecting with pathways that include proteins like Bcl-2 which are essential in regulating cell death and survival equilibrium.