Abcam, ab264887, Human TANK (TRAF2) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TANK KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TANK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TANK (TRAF family member-associated NF-kappa-B activator) and TRAF2 (TNF receptor-associated factor 2) are proteins involved in the regulation of immune responses. TANK also known as I-TRAF is a protein with a mass of approximately 49 kDa. TRAF2 has a mass of approximately 56 kDa. These proteins express in various immune cells including T and B lymphocytes. TANK interacts with TRAF2 to mediate downstream signaling pathways essential for immunity.
Biological function summary
The TANK/TRAF2 complex plays a role in modulating the NF-kappa-B signaling pathway which is important for inflammation and immune responses. This complex also contributes to cellular responses to stress and infection. TANK acts as an adaptor protein that interacts with TRAF2 to relay signals from upstream receptors ensuring proper immune function. Additionally TANK inhibits excessive activation of NF-kappa-B maintaining a balanced immune response.
Pathways
As it relates to regulating immune functions TANK/TRAF2 is pivotal in the NF-kappa-B and MAPK signaling pathways. In the NF-kappa-B pathway TRAF2 helps mediate the activation of downstream kinases which are critical for the translocation of NF-kappa-B to the nucleus. TANK works alongside other signaling molecules like TRAF3 and TRAF6 integrating signals that lead to gene transcription involved in immune response and cell survival.
The dysfunction of TANK/TRAF2 differs from normal regulatory controls it links to auto-inflammatory diseases and certain cancers. In autoimmune disorders such as systemic lupus erythematosus aberrant NF-kappa-B activity possibly influenced by TANK/TRAF2 leads to uncontrolled immune activation. In cancer particularly lymphomas altered TANK/TRAF2 signaling correlates with the unchecked proliferation of cells. The protein's interplay with receptor-interacting protein 1 (RIP1) further emphasizes its role as disruptions in this interaction are seen in pathological states.