Abcam, ab264893, Human USP40 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
USP40 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-USP40, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
USP40 also known as Ubiquitin Specific Peptidase 40 functions as a deubiquitinating enzyme. It belongs to a family of enzymes that remove ubiquitin from proteins. USP40 has a molecular mass of approximately 140 kDa. The expression of USP40 occurs mainly in tissues like the brain and testis. It lacks deubiquitinase activity on some substrates indicating specificity in its action compared to other ubiquitin proteases.
Biological function summary
The role of USP40 in cellular processes centers on regulating protein degradation. This regulation typically affects the stability and function of target proteins. It is not known to form a complex with other proteins but its action can still influence cellular homeostasis and stress responses indirectly. If not correctly regulated protein degradation can impact many cellular processes leading to varied biological outcomes.
Pathways
Several components of the ubiquitin-proteasome system involve USP40. This system plays an important role in maintaining protein quality control within cells. It interacts with proteins such as ubiquitin C-terminal hydrolase L1 (UCHL1) to modulate protein turnover and stability. Another pathway significantly influenced by USP40 is the autophagy-lysosome pathway where it impacts the recycling and degradation of cellular components.
Irregularities in USP40 have associations with neurodegenerative conditions like Parkinson's disease. In these contexts the dysfunction of protein degradation pathways contributes to the accumulation of misfolded proteins. USP40's association with Alzheimer's disease arises from its interaction with proteins involved in synaptic function and degradation processes like presenilin-1 (PSEN1). Enhancing the understanding of USP40 functions could provide insights into therapeutic strategies for these diseases.