Abcam, ab264924, Human SLC43A3 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SLC43A3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC43A3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SLC43A3 protein also known as ENBT3 functions as a transporter for nucleobases like adenine and hypoxanthine. It belongs to the SLC43 family specifically facilitating the movement of nucleobases across the cell membrane. This protein has an approximate molecular mass of 51 kDa and is expressed in various tissues including the liver kidney and placenta. SLC43A3 plays an important role in regulating nucleotide levels within cells and maintaining a balance necessary for many cellular processes.
Biological function summary
This protein ensures the uptake of essential nucleobases contributing to nucleotide biosynthesis and salvage pathways. SLC43A3 does not function as part of a larger protein complex. Its role in transporting nucleobases is significant for cellular RNA and DNA synthesis indicating its involvement in cellular proliferation and repair. This highlights its importance in cellular growth and energy metabolism.
Pathways
SLC43A3 actively contributes to the purine salvage pathway critical for recycling nucleobases back into nucleotides. The purine salvage pathway is integral for conserving energy in cells as it allows the reuse of preformed bases. SLC43A3 is closely related to proteins like HPRT1 and APRT which also participate in this pathway. These interactions enable the efficient functioning of nucleotide metabolism and synthesis within various cell types.
Alterations in SLC43A3 expression or function may contribute to conditions such as cancer and certain metabolic disorders. Cancer in particular often involves enhanced nucleotide synthesis and salvage pathways to support rapid cell division. SLC43A3 by adjusting nucleotide availability can influence the progression of such diseases. Its interaction with other nucleotide-related proteins can also complicate metabolic diseases. Recognizing these links is helpful for understanding the molecular basis of these diseases and potentially targeting SLC43A3 for therapeutic interventions.