Abcam, ab264940, Human PITPNB knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PITPNB KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PITPNB, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PITPNB also known as phosphatidylinositol transfer protein beta isoform plays a role mechanically in lipid transfer between membranes. It weighs around 32 kDa. You can find this protein expressed in various tissues including the brain liver and lungs. PITPNB facilitates lipid metabolism and signaling by transferring phosphatidylinositol and phosphatidylcholine between membranes.
Biological function summary
PITPNB ensures the proper distribution of lipids within cells for membrane composition and signaling activities. It participates in maintaining the balance of lipid composition among cell membranes. PITPNB does not function alone; it often acts as part of lipid transfer complexes collaborating with other proteins to execute its function properly.
Pathways
PITPNB plays an active role in phosphoinositide signaling and membrane trafficking pathways. These pathways are critical for cell signaling and membrane dynamics. PI4K2A and PI4KA are proteins related to these pathways and interact with PITPNB highlighting its contribution to complex intracellular signaling processes.
Defects in PITPNB have been linked to metabolic disorders and neurodegenerative diseases such as Alzheimer's. PITPNB is connected to APOE a protein involved in lipid metabolism and associated with Alzheimer's disease progression. Understanding the role of PITPNB in these conditions opens possibilities for therapeutic strategies targeting lipid transfer processes.