Abcam, ab264995, Human BRWD1 (WDR9) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
BRWD1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-BRWD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The WDR9/BRWD1 protein is also known by its alternate name BRWD1 and has a molecular mass of approximately 250 kDa. This protein shows expression in various tissues with higher levels observed in testis and certain brain regions. WDR9/BRWD1 is a member of the WD-repeat protein family and contains two bromodomains. Mechanically it plays a role in mediating protein-protein interactions aiding in chromatin organization and gene regulation.
Biological function summary
WDR9/BRWD1 functions in regulating transcriptional processes and is often part of nuclear chromatin complexes. It participates in gene expression important for spermatogenesis and neuronal differentiation. This protein interacts with other chromatin-modifying proteins contributing to the alteration of chromatin states and affecting cellular programs.
Pathways
Different regulatory and developmental processes involve WDR9/BRWD1. The protein plays roles within chromatin remodeling pathways. It engages with pathways such as histone modification and transcriptional regulation working alongside proteins like histones and other transcription factors which helps coordinate the accessibility of transcriptional machinery to DNA.
Abnormal WDR9/BRWD1 expression or mutations have connections to neurological and reproductive conditions. Studies link its dysregulation to intellectual disabilities and fertility issues. Its association with chromatin-modifying proteins implicates it in diseases where gene expression regulation plays a role such as certain cognitive disorders.