Abcam, ab265037, Human SLC7A6 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SLC7A6 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and Insertion of the selection cassette in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and Insertion of the selection cassette in exon 4,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC7A6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SLC7A6 also known as LAT-2 is a protein that functions as part of the heterodimeric amino acid transporter complex. It has a molecular mass of approximately 55 kDa. This protein is primarily expressed in the liver and kidney but it can also be found in other tissues such as the skeletal muscles and brain. SLC7A6 is involved in the transportation of neutral and cationic amino acids across cell membranes which is essential for maintaining cellular homeostasis.
Biological function summary
The SLC7A6 protein participates in amino acid transport across the plasma membrane and it forms a functional complex with the glycoprotein 4F2hc/CD98. This association is necessary for the optimal activity and localization of SLC7A6 on the cell surface. Its role in amino acid exchange impacts nutrient sensing and plays a critical function in cellular growth and repair processes. The protein is involved in maintaining amino acid balance which is fundamental in many physiological processes.
Pathways
Amino acid transporters like SLC7A6 are significant in metabolic and mTOR signaling pathways. These pathways influence cellular growth proliferation and metabolism. SLC7A6 is closely related to other transporters like SLC7A5 within these pathways catalyzing the bidirectional movement of large neutral amino acids. It ensures the availability of essential amino acids for protein synthesis and energy production affecting the overall metabolic state of the cell.
Altered expression of SLC7A6 is linked to metabolic disorders and cancer. Disruption in its function can lead to amino acid imbalances contributing to the development of liver diseases. Additionally cancer cells often show increased expression of amino acid transporters like SLC7A6 and SLC7A5 to fulfill the high nutrient demands of rapidly dividing cells indicating its importance in cancer metabolism and potential as a therapeutic target.