Abcam, ab265083, Human MPV17 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MPV17 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 4,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MPV17, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MPV17 also known as MPV17 mitochondrial inner membrane protein plays an important role in the integrity of mitochondrial DNA (mtDNA) maintenance. It is a 20 kDa protein found primarily in the inner mitochondrial membrane. Expression of MPV17 occurs in various tissues notably in the liver kidney and central nervous system. The protein localizes to mitochondria where it is involved in maintaining mitochondrial function.
Biological function summary
MPV17 affects several processes related to cellular energy production. It is part of a larger protein family involved in mitochondrial homeostasis. MPV17 contributes to the regulation of oxidative phosphorylation by modulating mitochondrial DNA content. This regulation assures proper function of the respiratory chain complexes required for cellular energy provision. Additionally its activity helps prevent mtDNA damage that may arise from oxidative stress.
Pathways
MPV17 influences energy metabolism pathways critical for cell viability. It interacts with the mitochondrial DNA maintenance pathway facilitating the replication and distribution of mtDNA within cells. MPV17 has functional associations with other proteins like POLG which is the catalytic subunit of the mitochondrial DNA polymerase responsible for replication and repair. This relationship ensures the stability and transcription of mitochondrial genome necessary for proper cellular energy management.
Mutations in MPV17 lead to conditions like mitochondrial DNA depletion syndrome (MDDS) and Navajo neurohepatopathy. MDDS results in reduced mtDNA levels affecting energy production and leading to severe liver and neurological disorders. The disease links MPV17 to other proteins involved in mitochondrial maintenance such as TWNK another protein necessary for mtDNA maintenance and expression. Understanding MPV17's role in these pathologies can provide insights into potential therapeutic strategies for corresponding mitochondrial disorders.