Abcam, ab265084, Human PKDCC (VLK) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PKDCC KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 32 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 32 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PKDCC, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VLK also known as V-set and transmembrane domain-containing protein E is a serine/threonine kinase with a molecular mass of approximately 54 kDa. It localizes mainly in the Golgi apparatus. Scientists detect VLK expression across various tissues including the heart testis and liver. This kinase executes post-translational modifications by phosphorylating intracellular proteins which can modulate their functions and interactions.
Biological function summary
VLK kinase activity impacts protein folding and trafficking within the Golgi apparatus. VLK associates with protein complexes involved in secretory pathways assisting cellular functions like protein secretion and cell surface expression. Research indicates that it contributes significantly to maintaining cellular homeostasis by ensuring proper protein maturation.
Pathways
VLK plays a role in the secretory pathway which is essential for the transport and delivery of proteins and lipids throughout the cell. It shows interactions with proteins like ABCD3 and VCP that are pivotal in vesicular transport and protein quality control. VLK also participates in the RAS signaling pathway linking it to cell growth and differentiation by affecting downstream targets.
Recent studies highlight VLK's involvement with neurodegenerative diseases and cancer. Altered VLK activity correlates with conditions such as Alzheimer's disease where phosphorylation imbalances disrupt neuron function. In cancer VLK is overexpressed leading to misregulated cell proliferation. Connections to proteins like tau and LATS1 further demonstrate VLK's relevance in these diseases influencing both progression and therapeutic targeting.