Abcam, ab265096, Human GANC knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
GANC KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp deletion in exon 11. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp deletion in exon 11,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-GANC, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The GANC gene encodes the enzyme Glucosidase Alpha Neutral C (GAN Glucosidase) also commonly known as neutral alpha-glucosidase C. It plays an important role in carbohydrate metabolism by hydrolyzing terminal non-reducing (14)-linked alpha-glucose residues to release alpha-glucose. The enzyme mass is approximately 106 kDa. GANC is widely expressed in human tissues with significant presence in the liver pancreas and intestine where it contributes to glucose homeostasis and nutrient absorption.
Biological function summary
This enzyme is important for carbohydrate digestion and glucose production. GANC is not known to form part of a larger protein complex but acts individually to catalyze the breakdown of polysaccharides and oligosaccharides. By converting these molecules into glucose it provides an immediate source of energy for cells thereby supporting cellular metabolism and overall organismal energy balance.
Pathways
The enzyme takes part in the carbohydrate metabolism pathway and the glycogen degradation pathway. These pathways are instrumental in energy production and storage management in cells. GANC works in conjunction with enzymes like glycogen phosphorylase and beta-amylase to regulate the conversion of stored glycogen into glucose ensuring proper energy supply during fasting and physical activity.
GANC associations include diabetes mellitus and glycogen storage disorders. Dysfunction or imbalance in GANC activity can lead to improper glucose regulation characteristic in diabetes where glucose metabolism is impaired. The enzyme collaborates with insulin and glucagon regulation mechanisms to maintain glucose homeostasis. In glycogen storage disorders improper degradation of glycogen can exacerbate energy supply issues highlighting the significance of GANC in maintaining normal glucose levels and metabolic functions.