Abcam, ab265113, Human TOR3A knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TOR3A KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 1 bp deletion in exon 1 and 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 1 bp deletion in exon 1 and 1 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TOR3A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TOR3A also known as torsin-3A is a member of the torsin family of proteins. It has a molecular weight of approximately 39 kDa. This protein mainly localizes to the endoplasmic reticulum and nucleus where it plays a role in maintaining cellular integrity. TOR3A is involved in the regulation of protein folding ensuring that proteins attain and maintain their correct conformation. Its expression is found in various tissues but is particularly notable in neural tissues where proper protein folding is imperative for cell function.
Biological function summary
The protein participates in processes related to maintaining cellular homeostasis. Although not a direct component of large protein complexes TOR3A interacts with molecular chaperones to assist in the correct folding and assembly of polypeptides. This function is foundational for maintaining cell survival and minimizing stress responses. The protein may interact with elements of the cell's quality control systems to facilitate the degradation of misfolded proteins.
Pathways
TOR3A takes part in cellular stress response and protein quality control pathways. It functions alongside other proteins like heat shock proteins (HSPs) to modulate the folding and repair of cellular proteins ensuring that they are correctly synthesized and functional. These pathways are key to cellular homeostasis helping cells to cope with stress and alterations in their environment.
TOR3A has links to certain neurological disorders where protein misfolding occurs. Abnormal functioning of TOR3A can result in the accumulation of misfolded proteins contributing to conditions such as neurodegenerative diseases like Alzheimer's. It may also relate to dystonia a movement disorder characterized by involuntary muscle contractions where its interaction with TOR1A or torsin-1A becomes significant. These associations highlight the importance of protein quality control mechanisms in preventing disease conditions related to neural tissues.