Abcam, ab265117, Human HNRNPDL knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
HNRNPDL KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 38 bp deletion in exon 1 and 47 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 38 bp deletion in exon 1 and 47 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-HNRNPDL, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HNRPDL also known as heterogeneous nuclear ribonucleoprotein D-like is a protein with a mass of about 42 kDa. It plays an important role in RNA processing activities like splicing transportation stabilization and degradation. The protein is part of the hnRNP family which contains versatile nucleic acid-binding proteins involved in various aspects of mRNA processing and metabolism. HNRPDL is expressed in multiple tissues including high levels in muscle heart and brain indicating its broad functional importance.
Biological function summary
HnRNP D-like protein participates in regulating gene expression post-transcriptionally. It often forms a complex with other hnRNP proteins enabling the modulation of RNA metabolic processes. This protein binds to RNA through its nucleic acid-binding domains influencing the mRNA stability and pre-mRNA processing. It affects various cellular processes by regulating gene transcripts vital for maintaining normal cellular functions.
Pathways
HnRNP D-like protein is part of key processes that influence mRNA splicing and transport. It is involved in the mRNA surveillance pathway that monitors and degrades abnormal mRNA molecules. HNRPDL also interacts with other proteins like HNRNPA1 in these pathways showing its importance in RNA regulation and cellular homeostasis. Its function in these pathways suggests it plays a role in ensuring the accuracy of mRNA synthesis and processing.
Altered HNRPDL function is linked to conditions such as myopathy and some neurodegenerative diseases. Mutations or dysregulation in hnRNP D-like protein can affect muscle tissues and might contribute to muscle degeneration disorders. Additionally its abnormal function may also relate to neurological disorders potentially through pathways that involve other hnRNP family members like HNRNPA1. Understanding its connection to these diseases could provide insights into potential therapeutic targets for related conditions.