Abcam, ab265156, Human PTPRS knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PTPRS KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 5 and 1 bp insertion in exon 5. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 5 and 1 bp insertion in exon 5,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PTPRS, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PTPRS also known as Protein Tyrosine Phosphatase Receptor Type S is an enzyme with a molecular mass of approximately 193 kDa. It operates as a receptor-type protein tyrosine phosphatase located on the cell surface. The protein consists of an extracellular domain a single transmembrane segment and an intracellular catalytic domain. Expression of PTPRS occurs in various tissues with significant presence in the central nervous system. It plays a role in cell signaling by dephosphorylating specific phosphotyrosine residues influencing cellular communication and signal transduction.
Biological function summary
Protein Tyrosine Phosphatase Receptor Type S affects multiple cellular processes. It is not part of a large protein complex but interacts with cell adhesion molecules and other proteins involved in cell growth and differentiation. PTPRS contributes to neural development and repair by influencing axonal growth and guidance. It regulates synaptic architecture by modulating signaling pathways that affect neural plasticity and regeneration.
Pathways
PTPRS is involved in neural-related signaling cascades modulating cellular responses. One notable pathway where PTPRS plays a significant role is the Src pathway which regulates processes related to growth and differentiation. PTPRS interacts with proteins like Src kinase family members impacting their activity through dephosphorylation. This modulation of kinase activity directly influences cellular outcomes such as proliferation and cell motility.
Protein Tyrosine Phosphatase Receptor Type S shows connections to neurodegenerative conditions and cancer. Altered PTPRS function associates with Alzheimer's disease where disrupted signaling affects neural pathways and contributes to the disease pathogenesis. In certain cancers abnormal PTPRS expression influences tumor progression by affecting cellular adhesion and migration. The protein interacts with cancer-related proteins such as integrins further contributing to disease development and progression through its regulatory mechanisms.