Abcam, ab265157, Human C9orf78 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
C9orf78 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-C9orf78, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C9orf78 also known as chromosome 9 open reading frame 78 is a protein with a molecular mass of approximately 24 kDa. It is expressed in various tissues but shows higher levels in the lung and reproductive organs. On a mechanical level C9orf78 is involved in intracellular processes where it interacts with other cellular components indicating its role in cellular signaling and regulation.
Biological function summary
C9orf78 plays a role in cellular homeostasis and regulation of metabolic processes. It acts as part of a complex that modulates cellular responses to stress and can influence gene expression. The protein's involvement in these cellular processes suggests that it serves as a mediator between cellular signaling pathways and the physical expression of cellular responses.
Pathways
C9orf78 contributes significantly to the AMPK signaling pathway and interacts with various proteins including LKB1. This association positions it as an important factor in energy metabolism helping the cell respond to energy depletion. Its role in the cellular energy balance connects it closely to upstream and downstream signals that control cellular growth and metabolism making it important for maintaining cellular function.
C9orf78 is associated with complex conditions like metabolic syndrome and cancer. It links to disease progression possibly through its relationship with AMPK as dysregulation in this pathway can lead to metabolic imbalance and growth irregularities. Additionally its interaction with proteins like LKB1 known for its tumor suppressive functions further emphasizes the importance of C9orf78 in maintaining normal cellular operations and its potential impact when malfunctioning.