Abcam, ab265175, Human SLC17A5 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SLC17A5 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 145 bp deletion in exon 1 and 49 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 145 bp deletion in exon 1 and 49 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC17A5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SLC17A5 also known as sialin is a protein encoded by the SLC17A5 gene. It is a lysosomal membrane protein with an approximate molecular mass of 55 kDa. This protein facilitates the transport of sialic acid from the lysosome to the cytoplasm following degradation of sialoglycoconjugates. It gets expressed in various tissues with highest expression levels noted in the brain and kidneys.
Biological function summary
The protein moves sialic acid a type of acidic sugar out of lysosomes important for cellular homeostasis. SLC17A5 transports not only free sialic acid but also ascorbate and other organic anions contributing to lysosomal function regulation. Though SLC17A5 functions mainly in lysosomes it can associate with membrane complexes that regulate ion transport across membranes.
Pathways
SLC17A5 is significant within lysosomal catabolism and membrane transport pathways. In these pathways it interacts with key proteins like neuraminidase which is involved in breaking down sialoglycoconjugates. The function of sialin in moving sialic acid affects these metabolic pathways and has an impact on maintaining cellular balance.
SLC17A5 has ties to free sialic acid storage disorders notably Salla disease and infantile sialic acid storage disorder (ISSD). These are neurodegenerative disorders linked to sialic acid accumulation caused by SLC17A5 malfunction. Mutations in this gene affect its transport function leading to these diseases. Additionally SLC17A5 relates to metabolic disorders by interacting with other transmembrane proteins potentially influencing disease progression.