Abcam, ab265206, Human ZCCHC17 (pNO40) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
ZCCHC17 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 3,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ZCCHC17, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PNO40 also known as RPLP0 is a ribosomal protein that plays a mechanical role in the ribonucleoprotein complex responsible for peptide synthesis. It has a molecular mass of about 34 kDa and is ubiquitously expressed across various human tissues especially in the cytoplasm and nucleolus. pNO40 functions as a structural component of the ribosome participating in the assembly and stability of the ribosomal large subunit. The protein is essential for the proper formation of ribosomal RNA and optimal ribosome performance during translation.
Biological function summary
PNO40 engages significantly in ribosomal biogenesis and protein synthesis where it anchors the essential phosphoprotein complex of the ribosome. Its presence ensures that translation occurs effectively influencing cellular protein production. This protein is not part of a complex by itself but often interacts with acidic ribosomal phosphoproteins including RPLP1 and RPLP2 which are important for ribosomal assembly and function. The activity of pNO40 ensures the ribosome's structural integrity critical for maintaining cellular homeostasis.
Pathways
When considering translational machinery pNO40 actively participates in the translation pathway ensuring efficient protein synthesis. It associates with the ribosome-related pathway where it interacts with proteins like EF-G (Elongation Factor G) that catalyze the translocation of the ribosome along mRNA. These interactions are key to understanding how protein synthesis influences cellular response to environmental changes. The functionality of pNO40 within these pathways reflects its foundational role in maintaining cellular functions.
The expression and function of pNO40 have implications for certain types of cancer and anemia. In cancer altered levels of pNO40 can lead to dysregulated protein synthesis contributing to rapid cellular proliferation. This protein interacts with dysregulated pathways that also involve tumor suppressor proteins like p53. In anemia impairment of pNO40 can affect ribosome assembly reducing erythropoiesis. Understanding these interactions is important for potential therapeutic strategies targeting ribosomal biogenesis.