Abcam, ab265222, Human REXO4 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
REXO4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-REXO4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
REXO4 also known as RNA exonuclease homolog 4 is a protein that plays a role in RNA processing. It has a molecular mass of approximately 33 kDa. REXO4 is found in the nucleolus and also distributes within the cytoplasm. This protein exhibits exonuclease activity participating in the degradation and/or processing of RNA molecules. By cutting RNA molecules it facilitates the regulation of gene expression which is important for proper cellular function and growth.
Biological function summary
RNA exonucleases like REXO4 contribute to RNA surveillance processes that maintain the quality of RNA. REXO4 might function as part of a larger complex tasked with the maturation and degradation of RNA. These activities are essential especially in the context of removing faulty RNA molecules that could cause deleterious effects if left unchecked in the cell. The precise role of REXO4 remains under investigation yet its contributions to the RNA life cycle are noteworthy.
Pathways
RNA processing and degradation involve REXO4 in essential molecular interactions. It participates in the RNA degradation pathway where it contributes to the turnover of RNA molecules ensuring cellular stability and homeostasis. This pathway often intersects with the nonsense-mediated decay pathway a critical quality control mechanism. Proteins such as UPF1 which are involved in the nonsense-mediated decay pathway might be functionally connected to REXO4 highlighting its importance in maintaining cellular RNA integrity.
Defects or dysregulation in the function of REXO4 may contribute to RNA-related conditions. Aberrant RNA processing has connections to disorders such as cancer. In certain cancers malfunctioning RNA degradation can lead to uncontrolled cell proliferation. Moreover faulty REXO4 activity might impact neurodegenerative diseases by affecting neuronal RNA metabolism. Interactions with proteins like TARDBP associated with such neurodegenerative disorders suggest that REXO4 may have broader implications in understanding RNA-linked diseases.