Abcam, ab265287, Human SMC1B (SMC1L2) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SMC1B KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 9 and 7 bp deletion in exon 9. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 9 and 7 bp deletion in exon 9,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SMC1B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMC1L2 also known as Structural Maintenance of Chromosomes 1-Like 2 is a protein involved in important processes in cell division. It is a part of the cohesin complex a structure that regulates the cohesion of sister chromatids during cell division. SMC1L2 has a molecular mass of approximately 155 kDa. This protein is expressed in various tissues with notable expression in testis and other reproductive organs reflecting its involvement in meiotic cell division.
Biological function summary
This protein plays a significant role in ensuring the accurate segregation of chromosomes during both mitosis and meiosis. SMC1L2 is an important component of the cohesin complex interacting closely with other proteins like SMC3 RAD21 and STAG3. By holding sister chromatids together it helps maintain genomic stability and prevents aneuploidy which can lead to compromised cell function or cell death if not regulated properly.
Pathways
SMC1L2 functions in critical cellular mechanisms such as the cell cycle and DNA repair pathways. The protein contributes to the mitotic cell cycle particularly in the regulation of sister chromatid cohesion an essential checkpoint for proper cell cycle progression. Furthermore SMC1L2 involvement in the DNA damage response highlights its connection with proteins like ATM and ATR which act as sensors and mediators of DNA repair mechanisms following genotoxic stress.
SMC1L2 mutations or malfunctions can be associated with reproductive issues and chromosomal instability disorders. For instance defects in this protein can lead to male infertility due to its role in spermatogenesis. Moreover the protein's dysfunction could contribute to cancer development by compromising genomic integrity. SMC1L2 may interact with altered p53 pathways as chromosomal stability is important in tumor suppression and prevention of cancerous cell growth.