Abcam, ab265309, Human MT1X knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MT1X KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MT1X, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MT1X also known as metallothionein-1X is a protein with a molecular mass of approximately 6-7 kDa. This protein belongs to the metallothionein family and plays an essential role in metal ion binding. MT1X primarily expresses in the liver kidneys and pancreas where it helps in stabilizing metal ions and detoxifying heavy metals. The structure of MT1X allows it to bind with metals like zinc and cadmium assisting in maintaining metal homeostasis.
Biological function summary
Metallothionein-1X acts as a protective agent against oxidative damage by binding and sequestering metal ions therefore preventing the generation of harmful reactive oxygen species. It does not form part of a complex independently but interacts with other proteins like metallothionein-2A to enhance cellular antioxidant defense. MT1X regulates metal ion concentrations within cells and contributes to the detoxification process safeguarding cells against metal-induced cytotoxicity.
Pathways
MT1X functions within pathways related to metal ion metabolism and detoxification processes. In the context of metal ion metabolism it interacts closely with proteins such as zinc transporter proteins maintaining optimal zinc levels in cells. MT1X also associates with the oxidative stress response pathway where it collaborates with proteins like glutathione to mitigate damage from oxidative stress.
Alterations in MT1X expression link to disorders like cancer and neurodegenerative diseases. In cancer an altered MT1X expression can impact cellular redox state and metal ion balance contributing to tumor progression. Its potential interplay with proteins like metallothionein-2A may influence cellular responses to oxidative stress in neurodegenerative diseases affecting neuronal survival and function. Understanding MT1X relationships with these proteins might offer insights into the pathogenesis and therapeutic strategies for these disorders.