Abcam, ab265351, Human LAMTOR1 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
LAMTOR1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 4 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 4 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LAMTOR1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LAMTOR1 also known as p18 is a protein with a mass of approximately 18 kDa. It functions mechanically as a scaffold protein located on the late endosome/lysosome membrane. The protein's primary role involves recruiting and stabilizing the other members of the Ragulator complex. LAMTOR1 is expressed in various tissues with significant levels in immune and neuronal cells where it supports cellular growth and immune responses.
Biological function summary
LAMTOR1 acts as a part of the highly-conserved Ragulator complex. The Ragulator complex is vital for the activation of the mechanistic target of rapamycin complex 1 (mTORC1) in response to amino acids. LAMTOR1's interaction with other components of the complex such as LAMTOR2 and LAMTOR3 facilitates the localization and activation of mTORC1 on the lysosomal surface. This activation promotes anabolic processes including protein lipid and nucleotide synthesis.
Pathways
LAMTOR1 plays an important role in the mTOR signaling pathway. This pathway regulates cell growth proliferation and metabolism. LAMTOR1's involvement with Rag proteins assists in sensing amino acid levels thereby influencing the mTORC1 pathway activation. Additionally LAMTOR1-related pathways intersect with the AMPK signaling pathway where it functionally interacts with proteins that control cellular energy homeostasis.
LAMTOR1's dysregulation has been associated with conditions like cancer and neurodegenerative disorders. Altered expression of LAMTOR1 can impact mTORC1 signaling leading to uncontrolled cell growth often observed in cancerous cells. In neurodegenerative disorders defects in mTORC1 pathway can cause neuronal cell damage. These conditions link LAMTOR1 to proteins like Rheb and TSC2 which are important for maintaining a balance in these signaling pathways.