Abcam, ab265369, Human LARP4B knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
LARP4B KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 149 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 149 bp insertion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LARP4B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LARP4B also known as La-related protein 4B is a protein of approximately 78 kDa. It is expressed in various tissues but shows higher expression in testis and liver. The protein contains an RNA-binding domain which indicates its role in RNA metabolism. LARP4B interacts with mRNA influencing its stability and translation. This protein belongs to the LARP family which is known for involvement in RNA-binding processes.
Biological function summary
LARP4B plays a role in regulating mRNA stability and translation. It often cooperates with mRNA decay and translation initiation factors. LARP4B does not form a stable complex but associates transiently with other RNA-binding proteins to perform its function. Its ability to bind mRNA suggests involvement in gene expression post-transcriptionally impacting various cellular processes.
Pathways
LARP4B is implicated in the mTOR signaling pathway and the RNA processing pathway. The mTOR pathway is essential for cell growth and proliferation linking nutrient availability to these processes. LARP4B works in tandem with other proteins like LARP1 and EIF4E to modulate mRNA stability indirectly influencing cell growth through translation regulation.
LARP4B has a connection to cancer and neurological disorders. Abnormal expression of LARP4B has been observed in certain cancers suggesting a role in tumorigenesis. In neurological disorders differences in LARP4B expression levels have been associated with disease progression. The protein establishes a relationship with LARP1 in cancer contexts further exploring how altered mRNA translation contributes to these diseases.