Abcam, ab265408, Human TRAF5 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TRAF5 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TRAF5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRAF5 known as TNF receptor-associated factor 5 plays a role in cellular signaling pathways. This protein has a molecular mass of approximately 64 kDa. It is expressed in various tissues including lymphoid tissues and cells of the immune system. TRAF5 acts in signaling downstream of certain receptors like TNF receptor superfamily where it functions as an adapter protein that mediates signal transduction by interacting with other signaling molecules.
Biological function summary
TRAF5 influences immune cell functions and inflammatory responses. It forms part of a larger protein complex that regulates the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway. Through this complex TRAF5 affects the transcription of genes involved in immune responses and cell survival. By modulating these processes TRAF5 helps maintain immune homeostasis and ensures proper immune response.
Pathways
TRAF5 is involved in signaling pathways such as NF-κB and JNK (c-Jun N-terminal kinase) pathways. TRAF5 interacts with proteins like TRAF2 and RIPK1 to propagate signals that lead to activation of these pathways. NF-κB pathway regulates inflammation and immune responses while JNK pathway plays a part in stress responses and apoptosis. The interaction of TRAF5 with these proteins and pathways positions it as an integral part of the immune signaling network.
TRAF5 associates with autoimmune diseases including rheumatoid arthritis and lupus. Abnormal signaling through TRAF5 can lead to dysregulated immune responses contributing to these conditions. In rheumatoid arthritis for example TRAF5 may abnormally activate NF-κB pathway leading to enhanced inflammation. Moreover the interaction between TRAF5 and other proteins like TRAF1 influences the development of these diseases by affecting signal transduction which is important for understanding potential therapeutic targets in these disorders.